The Israeli Journal of Aquaculture – Bamidgeh 57(4), 2005, 264-270
 
EFFECTS OF MOLTING ON THE GROWTH OF PRIMARY CULTURED MUSCLE CELLS FROM MACROBRACHIUM NIPPONENSE AND PENAEUS VANNAMEI

Wei-Na Wang1*, An-Li Wang1, Dong-Hong Nu2, Yi-Chen Liu2, and Ru-Yong Sun1

1 College of Life Science, South China Normal University, Guangzhou 510631, People’s Republic of China
2 College of Life Science, Hebei University, Baoding 071002, People’s Republic of China
 
(Received 23.6.05, Accepted 21.10.05)

Key words: cell culture, Macrobrachium nipponense, molt, Penaeus vannamei, RNA:DNA ratio
 
 
Abstract



This study examined the effects of molting stage on the growth of primary-cultured cells from the shrimps Macrobrachium nipponense and Penaeus vannamei. Muscle cells in different molting stages were cultured in L-15 medium, supplemented with 1g/l glucose,1g/l MgCl2,1 g/l CaCl2,100 IU/ml penicillin, and 100 µg/ml streptomycin. Results showed that cells cultured in the early postmolt stage grew best. They had an unfolding cell shape, a compact monolayer, a high rate of coverage, and the highest RNA:DNA ratio. Differences among early postmolt, premolt, and intermolt cells were significant ( p<0.05).


* Corresponding author. E-mail: weina63@yahoo.com.cn 

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