The Israeli Journal of Aquaculture Bamidgeh 57(2), 2005, 125-130        
 
EFFECT OF FREEZING TECHNIQUES, EXTENDERS AND CRYOPROTECTANTS ON THE FERTILIZATION RATE OF FROZEN RAINBOW TROUT (ONCORHYNCHUS MYKISS) SPERM

Yusuf Bozkurt1*, Ergun Akcay2, Necmettin Tekin2 and Selcuk Secer3

1Department of Aquaculture, Faculty of Fisheries, Mustafa Kemal University, 31040 Hatay, Turkey

2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary, Ankara University, 06110 Ankara, Turkey

3 Department of Fisheries and Aquaculture, Faculty of Agriculture, Ankara University, 06110 Ankara, Turkey
 
 
(Received 8.12.04, Accepted 14.3.05)
 

Key words: cryopreservation, extender, fertilization, Oncorhynchus mykiss , pellet, sperm, straw
 
 
Abstract


Rainbow trout (Oncorhynchus mykiss)sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in their own extenders at 30oC for 30 s. For both freezing methods, the best fertilization results were obtained with extender III containing 15% DMSO, 52.3% (56.3% of the control) for sperm frozen in straws and 48.4% (52.4% of the control) for sperm frozen as pellets. The interaction between extender and cryoprotectant was statistically significant ( p<0.05).


*Corresponding author. E-mail: yfbozkurt@mku.edu.tr

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