The Israeli Journal of Aquaculture Bamidgeh 57(1), 2005, 39-48

 
USE OF GLUCAN FROM SACCHAROMYCES CEREVISIAE AS 
AN IMMUNOSTIMULANT IN CARP: IMPACT ON HEMATOLOGY, 
PHAGOCYTE FUNCTION, AND INFECTION WITH 
AEROMONAS HYDROPHILA 


V. Selvaraj1*, K. Sampath2 and V. Sekar3* 
 

1 Spic Research and Development, Spic Limited, Tuticorin 628 005, Tamilnadu, India
 
2 Department of Zoology, V.O.C. College, Tuticorin 628 008, India 

3 Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, 
Madurai 625021, India 

(Received 13.3.04, Accepted 12.1.05) 


Key words: carp, leukocytes, macrophage, monocytes, NBT assay, neutrophils, opsonin, 
phagocytes 



Abstract


Glucan was extracted from yeast (Saccharomyces cerevisiae)and subjected to chemical analyses. Thin layer chromatography (TLC) revealed that glucan is primarily composed of glucose units and contains 97.2% glucose. Infrared spectrum showed a major band at 883/cm, suggesting that glucan is linked to b-glycosidic. Intraperitoneal administration of different doses of glucan enhanced the survival rate of carp (Cyprinus carpio) infected by Aeromonas hydrophila. When a concentration above 500 mg glucan per fish was administered, survival relative to the uninfected control was 100%. The total leukocyte count and neutrophil and monocyte populations increased after administration of the glucan. The maximum effect occurred on day 6 after injection. Macrophages of compound-administrated test animals significantly increased production of superoxide anions, efficiently killing the bacterial pathogen. This study showed that glucan can be effective in inducing non-specific cellular defense mechanism in carp.  


* Corresponding authors. V. Selvaraj, SGS Life Science, SGS India Pvt. Ltd. TICEL Bio Park Ltd, 
   Taramani Road, Taramani, Chennai-600113, Tamilnadu, India; e-mail: sselvarajv@yahoo.co.in
   V. Sekar, tel.: 91-452-2458684; e-mail: sekarpsb2001@yahoo.com

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