The Israeli Journal of Aquaculture Bamidgeh 56(4), 2004, 274-280

 
CORRELATION BETWEEN BIOCHEMICAL AND 
SPERMATOLOGICAL PARAMETERS IN RAINBOW TROUT 
(ONCORHYNCHUS MYKISS) SEMEN 


Selcuk Secer1, Necmettin Tekin2, Yusuf Bozkurt1*, Neslihan Bukan3 and Ergun Akcay2 

1 Department of Fisheries and Aquaculture, Faculty of Agriculture, Ankara University, 
06110 Diskapi, Ankara, Turkey 

2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary, 
Ankara University, 06110 Diskapi, Ankara, Turkey 

3 Department of Biochemistry, Faculty of Medicine, Gazi University, 06510 Besevler, 
Ankara, Turkey 


(Received 13.12.03, Accepted 7.6.04) 


Key words: Oncorhynchus mykiss, seminal plasma, spermatological parameters, spermatozoa, 
sperm motility, spawning season



Abstract


Levels of biochemical parameters in seminal plasma and physical parameters of rainbow trout (Oncorhynchus mykiss) semen were determined and compared. Seminal plasma contained 80.5131.48 mmol/l Na+, 46.2112.58 mmol/l K+, 4.651.57 mg/dl Ca2+, 3.481.18 mEq/l Mg2+, 112.525.93 mmol/l Cl-, 1.330.76 mg/dl glucose, 0.150.09 g/dl protein, 2.552.47 mg/dl cholesterol, 8.02.84 mg/dl triglyceride, and 31.6540.78 mg/dl urea. Semen volume was 19.921.43 ml, spermatozoa motility 60.030.09%, duration of spermatozoa movement 174.01.82 s, spermatozoa concentration 1.520.70 x 109/ml, total spermatozoa number 44.2564.59 x 109, and pH 6.70.25. There were significant positive correlations between semen volume and Na+ (r = 0.667, p<0.05), total spermatozoa number and semen volume (r = 0.977, p<0.01), total spermatozoa number and spermatozoa concentration (r = 0.652, p<0.05), Na+ and Cl- (r = 0.733, p<0.05), Na+ and triglyceride (r = 0.632, p<0.05), K+ and protein (r = 0.728, p<0.05), and Ca2+ and protein (r = 0.685, p<0.05). Significant negative correlations were found between Cl- and glucose (r = - 1.00, p<0.05), and triglyceride and urea (r = -0.716, p<0.05). Consequently, although there were 
no definite correlations between biochemical and spermatological parameters, it was observed that a higher Na+ content has a positive effect on semen volume.


* Corresponding author. E-mail: ybozkurt@agri.ankara.edu.tr

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