The Israeli Journal of Aquaculture – Bamidgeh
56(3), 2004, 200-208
DIETARY HISTIDINE REQUIREMENT OF FINGERLING INDIAN
MAJOR CARP, LABEO ROHITA (HAMILTON)
Shabihul Fatma Abidi and Mukhtar A. Khan*
Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University,
Aligarh, 202 002 UP, India
(Received 17.9.03, Accepted 13.3.04)
Key words: dietary histidine requirement, fingerlings, Labeo rohita
A 6-week growth trial was conducted to assess the dietary histidine requirement of fingerling
Indian major carp, Labeo rohita (3.50±0.04 cm; 0.40±0.02 g) by using amino acid test diets (40%
crude protein; 4.28 kcal/g) containing casein, gelatin, and L-crystalline amino acids. Diets with
graded levels of histidine (0.25, 0.50, 0.75, 1.00, 1.25, and 1.50% of the diet) were fed to triplicate
groups of fingerlings at 5% of their body weight divided into two feedings (07:00 and 17:30).
Live weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio were significantly
(p<0.05) affected by dietary histidine concentration. Weight gain and conversion efficiencies
were best at 0.75% dietary histidine. Whole body protein content was highest and moisture
and fat were lowest in the 0.75% dietary histidine treatment while whole body ash was the
same at all treatment levels. Second-degree polynomial regression analysis of the live weight
gain and feed conversion ratio resulted in more accurate histidine requirement estimates of 0.90
and 0.82% of the dry diet, respectively, corresponding to 2.25 and 2.05% of the dietary protein.
It is recommended that dietary histidine be included at a level of 0.82% of the feed, corresponding
to 2.05% of the dietary protein, for optimal growth in L. rohita fingerlings.
* Corresponding author. E-mail: firstname.lastname@example.org