The Israeli Journal of Aquaculture Bamidgeh 56(1), 2004, 35-44.

EFFECTS OF DICHLORVOS AND CHARACTERIZATION OF MICROSOMAL NADPH-CYTOCHROME P450 AND NADH-FERRICYANIDE REDUCTASE IN RAINBOW TROUT
(ONCORHYNCHUS MYKISS) LIVER


Tevfik Ozen*, Halil Korkmaz

Department of Chemistry, Faculty of Arts and Sciences, Ondokuz Mayis University, 55139, Kurupelit, Samsun, Turkey

(Received 28.5.03, Accepted 23.8.03)


Key words: DDVP, microsomes, NADPH-cytochrome P450 reductase, NADH-ferricyanide reductase, Oncorhynchus mykiss


Abstract

The kinetics of oxidation of rainbow trout (Oncorhynchus mykiss) NADPH-cytochrome P450 reductase (NCPR) by cytochrome-c and NADH-ferricyanide reductase (NFR) by K3Fe(CN)6 were studied. NCPR and NFR activities from liver microsomes of rainbow trout (Oncorhynchus mykiss) had a specific activity of 0.0246 and 0.0350 nmoles/min/mg protein, respectively. The maximal rate of NCPR reaction was found at cytochrome-c concentrations above 2.1 mM and in the presence of cyt c. Vmax and Km were 0.0083 nmoles/min/mg and 1.14 mM. The maximal rate of NFR reaction was at K3Fe(CN)6 concentrations higher than 2.0 mM and in the presence of K3Fe(CN)6. Vmax and Km values were 1.04 nmoles/min/mg and 0.352 mM, respectively. The inhibition by dichlorvos (DDVP) on NCPR and NFR activity was not exhibited over the range 1.0-5.0 mM DDVP.

* Corresponding author. Tel.: +90-362-4576020, ext. 5199, fax: +90-362-4578081,
e-mail: tevfikoz@omu.edu.tr

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