The Israeli Journal of Aquaculture - Bamidgeh 55(3), 2003, 208-212

SHORT COMMUNICATION:
CRYOPRESERVATION OF RAINBOW TROUT
(ONCORHYNCHUS MYKISS) SEMEN


Necmettin Tekin¹*, Selçuk Secer², Ergun Akcay¹, Yusuf Bozkurt²

1 Department of Reproduction and Artificial Insemination, Faculty of Veterinary, Ankara University, 06110 Diskapi, Ankara, Turkey

2 Department of Fisheries and Aquaculture, Faculty of Agriculture, Ankara University, 06110 Diskapi, Ankara, Turkey



(Received 6.12.02, Accepted 9.7.03)

Key words: cryopreservation, extender, fertilization, Oncorhynchus mykiss, semen, spermatological properties


Abstract

Rainbow trout semen was diluted in one of three extenders (1:3 v/v) containing egg yolk (7-10%) and 10% DMSO as cryoprotectants. The diluted semen was packaged in 0.5 ml straws and frozen in liquid nitrogen (LN2) vapor. For thawing, the straws were immersed in a water bath for 30 sec at 30C. The best post-thaw motility and fertilization results, 56.675.77% and 80.516.01% respectively, were obtained when a glucose based extender was used.


*Corresponding author: e-mail: ntekin@veterinary.ankara.edu.tr

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