The Israeli Journal of Aquaculture - Bamidgeh 53(3-4), 2001, 89-100

PRODUCTION OF HETEROZYGOUS AND HOMOZYGOUS CLONES
OF COMMON CARP (CYPRINUS CARPIO L.): EVIDENCE FROM
DNA FINGERPRINTING AND MIXED LEUKOCYTE REACTION

Naomi Ben-Dom and Nina B.Cherfas
Agricultural Research Organization, Institute of Animal Science,
Department of Aquaculture, Dor, DN Hof Hacarmel 30820, Israel


Boris Gomelsky
Ministry of Agriculture and Rural Development, Department of Fisheries,
Fish and Aquaculture Research Station, Dor, DN Hof Hacarmel 30820, Israel


Ramy R.Avtalion
Laboratory of Fish Immunology and Genetics, Faculty of Life Sciences,
Bar Ilan University, Ramat Gan 52900, Israel


Boaz Moav
Department of Zoology, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel

Gideon Hulata
Agricultural Research Organization, Institute of Animal Science,
Department of Aquaculture, PO Box 6, Beit Dagan 50250, Israel
Fax: 972-3-9605667, e-mail: vlaqua@volcani.agri.gov.il


(Received 10.6.01, Accepted 23.7.01)

Key words: common carp, Cyprinus carpio, DNA fingerprinting, heterozygosity, homozygosity, mixed leukocyte reaction

Abstract

The production of two heterozygous and one homozygous clones of the Israeli Dor-70 line of common carp (Cyprinus carpio L.) was tested using DNA fingerprinting (DFP)and mixed leukocyte reaction (MLR) analysis.The clones were obtained in large-scale production from mitotic gynogenetic females and males (sex-inversed). MLR analysis was used to examine major histocompatibility complex (MHC) antigens in individuals. DFP provided evidence concerning identity in the whole genome. Both methods showed that individuals within a progeny were identical, confirming genetic uniformity within each clone and showing that no paternal DNA was transmitted during mitotic and meiotic gynogenesis. The results indicated that uniformity of DFP in progeny of the homozygous clone is obtained only when the mother is fully homozygous, and in the heterozygous clone only if both parents are fully homozygous. The results of both methods provide evidence, though indirect, that the mitotic gynogenetic parents of these fish (mother of the homozygous clone, mother and father of the heterozygous clones) were fully homozygous.

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