The Israeli Journal of Aquaculture - Bamidgeh 53(3-4), 2001, 110-114

IMMUNOGENICITY OF AEROMONAS SALMONICIDA
A-PROTEIN IN GOLDFISH (CARASSIUS AURATUS L.)

M. Sinyakov¹, M. Dror¹, S. Margel ² and R. R. Avtalion¹*

1 Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900, Israel
*Corresponding author: R. R. Avtalion avtalr@mail.biu.ac.il

2 Department of Chemistry, Bar Ilan University, Ramat Gan 52900, Israel


(Received 14.10.00,Accepted 8.11.01)

Key words: Aeromonas salmonicida, A-layer protein immunogenicity, goldfish

Abstract

Antigenicity of the surface A-layer protein of an atypical Aeromonas salmonicida strain was tested in goldfish following immunization with various A-protein (AP) antigenic preparations (soluble form, alum-precipitated, conjugated with polyacrolein nanoparticles, or bacterin). ELISA revealed innate specific anti-AP antibody activity in all fish on day 0, while no significant antibody enhancement was seen on days 21 or 42 following the primary immunization. A second stimulation with soluble AP resulted in a clear response in groups primed with bacterin and alum-precipitated AP but no response in those primed with soluble AP or AP-nanoparticles conjugate. The implication of these results for vaccine design is discussed.

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