The Israeli Journal of Aquaculture - Bamidgeh 52(4), 2000, 151-158

MOLECULAR DNA VARIATION IN KOI (CYPRINUS CARPIO)
OF VARIOUS COLOR PATTERNS

K. Jackson ¹,² , D. Goldberg ¹,² , Y. Yehuda ¹ and G. Degani ¹, ², ³

1 MIGAL - Galilee Technological Center, Kiryat Shmona 10200, Israel
2 Tel Hai College, School of Science and Technology, Israel
3 Golan Research Institute, University of Haifa, Israel

(Received 19.5.99, Accepted 2.12.00)

Abstract

Molecular DNA variations in koi (Cyprinus carpio) of various color patterns were studied by random amplified polymorphic DNA (RAPD) PCR. For DNA examination, nine strains were divided into two groups. Group one consisted of sanke, showa, yamatunishaki, kohaku and gold ohgon. Group two consisted of platinum ohgon, orenji ohgon, nezu ohgon and kohaku. Four (ZG4, ZG8, I18 and 18498) of the 29 primers tested for this study amplified the DNA, resulting in a polymorphic pattern.

Genetic similarity between strains, as calculated by bandsharing (BS), showed an identical pattern in yamatunishaki and sanke (99%) and close similarity between ohgon and sanke (94%), ohgon (gold) and kohaku (88%), and sanke and kohaku (94%) with primers ZG4, ZG8 and I18. Using these same primers, the BS of showa, compared with all the other strains, varied from 80 to 88%. Much higher genetic variation (low BS) was found in the comparisons between platinum ohgon, orenji ohgon, nezu ohgon and kohaku. In this case, the BS value varied from 28 to 75%.

home