The lsraeli Joumal of Aquaculture - Bamidgeh 50(3), 1998, 128-133

EXPERIMENTAL INFECTION OF KURUMA PRAWN
(PENAEUS JAPONICUS) WITH VIBRIO PENAEICIDA


Leobert D. de la Peńa

Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan 5021, Ioilo, Philippines

Toshihiro Naka and Kiyokuni Muroga

Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima 739-8528. Japan

(Received 22.2. 98, Accepted 6.6.98)

Abstract

To estimate the portal of entry of Vibrio penaeicida in kuruma prawn (Penaeus japonicus), immersion and oral infection experiments were conducted with pre-treated and intact prawns. In immersion challenge, V. penaeicida produced 30% mortality in the shell-removed group at both 108 and 107 CFU/ml doses while in the eye-cut group, mortalities were 44% and 13% at 106 and 108 CPU/ml, respectively. In the intact group, 10% mortality was observed at 108 CFU/ml and no mortality at 107 CPU/ml after two weeks. The pathogen produced high mortalities in the orally incubated group, the median lethal dose (LD50) being 103-104 CPU/animal. In the groups given V. penaeicida inoculated kuruma prawn meat and V. penaeicida-contaminated commercial pellets, mortalities within 60 days were 10% and 20%, respectively. These results suggest that the main portal of entry of V. penaeicida is the gastro-intestinal tract although the cuticle (epidermis) or wounds can also be portals.


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